Technology

What is Molecular Viability Testing (MVT) Technology?

Exponentially more sensitive than all current pathogen detection products, MVT technology is an innovative method for detecting viable bacterial pathogens versus non-viable pathogens or residual nucleic acids that can lead to false positives. This is a common problem in existing commercial nucleic acid detection systems.

Why is the MVT System Superior to Other Nucleic Acid Detection Systems?

Pre-ribosomal RNAs (Pre-rRNA) is the basis of our patent-pending, highly sensitive and specific system. Pre-rRNAs are produced in high copy numbers very quickly after viable bacterial cells experience optimal growth conditions. Non-viable cells do not produce Pre-rRNAs. By exploiting this fact, our system does not produce false positives and is less likely to produce false negatives because of the high copy number and orders-of-magnitude increased sensitivity with Pre-rRNAs versus current commercial DNA or RNA detection systems. False positives and false negatives are a costly reality in most commercial nucleic acid detection systems.



Scientific Publications

Dead or Alive: Molecular Assessment of Microbial Viability. Cangelosi GA1, Meschke JS2

Biosynthetic Enhancement of the Detection of Bacteria by the Polymerase Chain Reaction Julie S. Do,1 Kris M. Weigel,2 John S. Meschke,2 and Gerard A. Cangelosi2,* Nancy E. Freitag, Editor

Molecular Viability Testing of Bacterial Pathogens from a Complex Human Sample Matrix Kris M. Weigel, Kelly L. Jones, Julie S. Do, Jody Melton Witt, Jae-Hyun Chung, Christian Valcke, Gerard A. Cangelosi

Poster: Molecular Viability Testing of Bacterial Pathogens Kris M. Meigel, Kelly L. Jones, Jody Melton Witt, Julie S. Do, Christian Valcke, and Gerard A.Cangelosi, Seattle Biomed, Novartis Vaccines and Diagnostics and AttoDx, Inc. 

Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis Gerard A. Cangelosi, Kris M. Weigel, Clarita Lefthand-Begay, and John S. Meschke, Seattle Biomed, Dept. of Global Health, University of Washington, Dept. of Environmental and Occupational Health Sciences, University of Washington